Briefly, polySia avDP20 was oxidized to an aldehyde by sodium metaperiodate, and then hydrazide coupled biotin (Thermo Scientific) was conjugated to form a hydrazone bond. If you liked this information…
In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion…
Specificity of carbohydrate binding by AAV1 capsids on a glycan array. Interestingly, an older version of the array, containing glycans immobilized as biotinylated glycosides on a 384-well streptavidin-coated plate, was…
HepG2 (A) and Pro-5 (B) cells were transduced with a constant amount of AAV1-luc or AAV6-luc vectors in the presence of a 200-fold-excess amount of competing AAV1, AAV6, or AAV2…
Which application/end-user or product type may seek incremental growth prospects? AAV6 binding appears to be less affected by neuraminidase compared with AAV1 binding, suggesting that AAV6 may also bind to…
To confirm correct sequences in plasmids constructed, the plasmids can be analyzed by standard techniques such as by restriction endonuclease digestion, and/or sequencing according to known methods. Yeast Integrating plasmids…
Given the structural resemblance of RgNanOx to YjhC, it is likely that the E. coli oxidoreductase also uses the same mechanism of action for the reversible conversion of 2,7-anhydro-Neu5Ac to…
What is the Type of Sialic Acid market? In terms of production side, this report researches the Sialic Acid production, growth rate, market share by manufacturers and by region (region…
Background was washed out with deionized water and gel was scanned with the default instrument settings for analysis (Lexmark CX727 scanner). Inhibition of glycolipid synthesis was carried out as described…
Dendritic cells (DCs) play an essential role in immunity against bacteria by phagocytosis and by eliciting adaptive immune responses. The phagocytosis of different pathogenic E. coli isolates was also enhanced…
